QSPR Study on Enhanced Resonance Light Scattering of Dye-Human Serum Albumin

Chapter 3: COMPARING THE SELF- AGGREGATION POTENTIAL OF BOVINE SERUM ALBUMIN TO HUMAN SERUM ALBUMIN USING LASER LIGHT SCATTERING

Light Scattering Investigation of Charge Fluctuations in Isoionic Serum Albumin

Light scattering investigations have been carried out on Xrmour bovine plasma albumin (BSA), bovine serum mercaptalbumin (BMA) and human serum mercaptalbumin (HMA) in their isoelectric region. All three proteins were deionized by passing over an ion-exchange column. In the case of salt free aqueous solutions it was found that the function H(C,/ A+) is close to linear in the square root of prote...

Bovine Serum Albumin–dye Binding

The interaction of the dye reactive bright red 6C with bovine serum albumin has been investigated. Formation of a complex between reactive bright red 6C and bovine serum albumin was detected by use of absorption difference spectroscopy. The curves obtained by titration of the protein against the dye were recorded at different pH and ionic strength of the solution. An unusual form of titration c...

The Effects of Acetaminophen on Human Serum Albumin (HSA)

Thermal conformational changes in human serum albumin (HSA) in present with a 10 mM phosphate buffer, at pH=7 have been investigated via circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature in a range of 25oC to 55oC could induce a reversible conformational change in the structure of HSA. The HSA phase transition corresponds to the physiological and patho...

Nuclear magnetic resonance study of water sorbed on serum albumin.

The nuclear magnetic resonance line widths of water sorbed on solid bovine serum albumin have been determined as functions of temperature and water content of the albumin samples. The results indicate that, depending on its amount, the sorbed water may exist in three different states or arrangements. The first of these involves direct binding of water molecules to available polar groups on the ...